- Title
- Expression of sucrose synthase in the developing endosperm is essential for early seed development in cotton
- Creator
- Ruan, Yong-Ling; Llewellyn, Danny J.; Liu, Quing; Xu, S.; Wu, Li-Min; Wang, Lu; Furbank, Robert T.
- Relation
- Functional Plant Biology Vol. 35, Issue 5, p. 382-393
- Publisher Link
- http://dx.doi.org/10.1071/FP08017
- Publisher
- CSIRO Publishing
- Resource Type
- journal article
- Date
- 2008
- Description
- Successful seed development requires coordinated interaction of the endosperm and embryo. In most dicotyledonous seeds, the endosperm is crushed and absorbed by the expanding embryo in the later stages of seed development. Little is known about the metabolic interaction between the two filial tissues early in seed development. We examd. the potential role of sucrose synthase (Sus) in the endosperm development of cotton. Sus was immunol. localised in the cellularising endosperm, but not in the heart-stage embryo at 10 days after anthesis. The activities of Sus and acid invertase were significantly higher in the endosperm than in the young embryos, which corresponded to a steep concn. difference in hexoses between the endosperm and the embryo. This observation indicates a role for the endosperm in generating hexoses for the development of the two filial tissues. Interestingly, Sus expression and starch deposition were spatially sepd. in the seeds. Silencing the expression of Sus in the endosperm using an RNAi approach led to the arrest of early seed development. Histochem. analyses revealed a significant redn. in cellulose and callose in the deformed endosperm cells of the Sus-suppressed seed. The data indicate a critical role of Sus in early seed development through regulation of endosperm formation.
- Subject
- embryo; gene silencing; Sus
- Identifier
- uon:5414
- Identifier
- http://hdl.handle.net/1959.13/43294
- Identifier
- ISSN:1445-4408
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